Determination of serum or plasma glucose on the "AutoAnalyzer II" by use of the hexokinase reaction.

Manual procedures based on these reactions and involving the direct addition of serum to a single reagent are commonly used as emergency procedures in routine clinical chemistry laboratories of hospitals. We have adapted one of these methods, “Glucose 500-Pack” (Calbiochem, P.O. Box 12087, San Diego, Calif. 92772) to an automated method on the “AutoAnalyzer II” (Technicon Instruments Corp., Tarrytown, N.Y. 10591). The AutoAnalyzer II is well suited to automation of this method because the sample blank can be measured simultaneously. In addition, smaller manifold pump tubes can be used with the AutoAnalyzer II colorimeter than were necessary in the AutoAnalyzer “N” methodologies, thus permitting use of about half the reagent volume per sample suggested in the reagent manufacturer’s instructions.